Download Cancer Stem Cells: Methods and Protocols written by Gianpaolo Papaccio, Vincenzo Desiderio in PDF format. This book is under the category Biology and bearing the isbn/isbn13 number 9781493974009/978-1493974009. You may reffer the table below for additional details of the book.
This detailed volume; Cancer Stem Cells: Methods and Protocols (PDF) gathers a comprehensive collection of protocols; methods; and procedures used for the characterization; identification; and selection of cancer stem cells. These include surface marker expression; side population; spheres formation; ALDH activity; and numerous others. Written for the highly successful Methods in Molecular Biology series; chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step; readily reproducible protocols; and expert tips and troubleshooting advice to avoid potential pitfalls.
Very practical and comprehensive; Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study.
Gianpaolo Papaccio, Vincenzo Desiderio
Table of contents
Table of contents :
Front Matter ….Pages i-xii
Introduction to Cancer Stem Cells: Past, Present, and Future (David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle et al.)….Pages 1-16
Surface Markers for the Identification of Cancer Stem Cells (Vinod Gopalan, Farhadul Islam, Alfred King-yin Lam)….Pages 17-29
The Role of CD44 and Cancer Stem Cells (Liang Wang, Xiangsheng Zuo, Keping Xie, Daoyan Wei)….Pages 31-42
Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay (Luigi Mele, Davide Liccardo, Virginia Tirino)….Pages 43-48
Isolation of Cancer Stem Cells by Side Population Method (Masayuki Shimoda, Masahide Ota, Yasunori Okada)….Pages 49-59
Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres (Pooja Mehta, Caymen Novak, Shreya Raghavan, Maria Ward, Geeta Mehta)….Pages 61-75
In Vitro Tumorigenic Assay: The Tumor Spheres Assay (Hui Wang, Anna M. Paczulla, Martina Konantz, Claudia Lengerke)….Pages 77-87
In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells (Vijayalakshmi Rajendran, Mayur Vilas Jain)….Pages 89-95
Xenograft as In Vivo Experimental Model (Manuela Porru, Luca Pompili, Carla Caruso, Carlo Leonetti)….Pages 97-105
How to Assess Drug Resistance in Cancer Stem Cells (Maria Laura De Angelis, Ruggero De Maria, Marta Baiocchi)….Pages 107-115
Tumor Tissue Analogs for the Assessment of Radioresistance in Cancer Stem Cells (Meenakshi Upreti)….Pages 117-128
Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML) (Anna Di Biase, Amanda K. Miles, Tarik Regad)….Pages 129-138
Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System (Magdalena E. Buczek, Stephen P. Reeder, Tarik Regad)….Pages 139-148
Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform (Hiromichi Kawasaki, Angela Lombardi, Michele Caraglia)….Pages 149-156
Assessing DNA Methylation in Cancer Stem Cells (Sudipto Das, Bruce Moran, Antoinette S. Perry)….Pages 157-178
Histones Acetylation and Cancer Stem Cells (CSCs) (Vivian Petersen Wagner, Manoela Domingues Martins, Rogerio Moraes Castilho)….Pages 179-193
Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods (Martina Intartaglia, Rosalaura Sabetta, Monica Gargiulo, Giovanna Roncador, Federica Zito Marino, Renato Franco)….Pages 195-211
Circulating Tumor Cells (Sebastián A. García, Jürgen Weitz, Sebastian Schölch)….Pages 213-219
Back Matter ….Pages 221-223
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