Cancer Stem Cells: Methods and Protocols


Download Cancer Stem Cells: Methods and Protocols written by Gianpaolo Papaccio, Vincenzo Desiderio in PDF format. This book is under the category Biology and bearing the isbn/isbn13 number 9781493974009/978-1493974009. You may reffer the table below for additional details of the book.

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This detailed volume; Cancer Stem Cells: Methods and Protocols (PDF) gathers a comprehensive collection of protocols; methods; and procedures used for the characterization; identification; and selection of cancer stem cells. These include surface marker expression; side population; spheres formation; ALDH activity; and numerous others. Written for the highly successful Methods in Molecular Biology series; chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step; readily reproducible protocols; and expert tips and troubleshooting advice to avoid potential pitfalls.

Very practical and comprehensive; Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study.

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Gianpaolo Papaccio, Vincenzo Desiderio






223 pages









Table of contents

Table of contents :
Front Matter ….Pages i-xii
Introduction to Cancer Stem Cells: Past, Present, and Future (David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle et al.)….Pages 1-16
Surface Markers for the Identification of Cancer Stem Cells (Vinod Gopalan, Farhadul Islam, Alfred King-yin Lam)….Pages 17-29
The Role of CD44 and Cancer Stem Cells (Liang Wang, Xiangsheng Zuo, Keping Xie, Daoyan Wei)….Pages 31-42
Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay (Luigi Mele, Davide Liccardo, Virginia Tirino)….Pages 43-48
Isolation of Cancer Stem Cells by Side Population Method (Masayuki Shimoda, Masahide Ota, Yasunori Okada)….Pages 49-59
Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres (Pooja Mehta, Caymen Novak, Shreya Raghavan, Maria Ward, Geeta Mehta)….Pages 61-75
In Vitro Tumorigenic Assay: The Tumor Spheres Assay (Hui Wang, Anna M. Paczulla, Martina Konantz, Claudia Lengerke)….Pages 77-87
In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells (Vijayalakshmi Rajendran, Mayur Vilas Jain)….Pages 89-95
Xenograft as In Vivo Experimental Model (Manuela Porru, Luca Pompili, Carla Caruso, Carlo Leonetti)….Pages 97-105
How to Assess Drug Resistance in Cancer Stem Cells (Maria Laura De Angelis, Ruggero De Maria, Marta Baiocchi)….Pages 107-115
Tumor Tissue Analogs for the Assessment of Radioresistance in Cancer Stem Cells (Meenakshi Upreti)….Pages 117-128
Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML) (Anna Di Biase, Amanda K. Miles, Tarik Regad)….Pages 129-138
Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System (Magdalena E. Buczek, Stephen P. Reeder, Tarik Regad)….Pages 139-148
Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform (Hiromichi Kawasaki, Angela Lombardi, Michele Caraglia)….Pages 149-156
Assessing DNA Methylation in Cancer Stem Cells (Sudipto Das, Bruce Moran, Antoinette S. Perry)….Pages 157-178
Histones Acetylation and Cancer Stem Cells (CSCs) (Vivian Petersen Wagner, Manoela Domingues Martins, Rogerio Moraes Castilho)….Pages 179-193
Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods (Martina Intartaglia, Rosalaura Sabetta, Monica Gargiulo, Giovanna Roncador, Federica Zito Marino, Renato Franco)….Pages 195-211
Circulating Tumor Cells (Sebastián A. García, Jürgen Weitz, Sebastian Schölch)….Pages 213-219
Back Matter ….Pages 221-223

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